Kerafast #EVU301，B Cell Activating Feeder Cell Line (EL-4-B5)，B细胞激活饲养层细胞（小鼠胸腺瘤连续细胞系）
Cell Line EL-4-B5 is a subclone of murine EL4 thymoma that is a bromo-deoxyufidine-resistant mutant, and can be used as a feeder layer to generate B cell cultures yielding human monoclonal antibodies.
IL-2 producing EL-4 mouse thymoma cells underwent mutagenesis with ethyl-methane-sulfonate resulting in fusion partners for T cell hybridomas. It was then discovered that the thymidine kinase-deficient and ouabaine-resistant clone EL-4 BurOUr 6.1 also activated murine and human B cells via cell contact. Subcloning produced the EL-4-B5 cells, which strongly activate B cells. EL4-B5 is grown with B cells to activate the B cells via direct cell contact to induce proliferation, differentiation, and secretion of antibody. The EL-4-B5 cell line offers a method for the generation of recombinant human mAbs from single antigen-specific B cell clones selected with fluorescent VLPs and can be used to generate human mAbs to many other viruses whose proteins can self-assemble into VLPs.
|Mouse thymoma continuous cell line
|Cells are both adherent and suspension. The suspension cells are the more healthy ones. Can tolerate large splits after line is established (volume ratios of 1:10, for example). Helper function is optimal when cells are in log phase growth. Keep density below 106cells per mL at all times
|RPMI-1640, 10% FBS heat inactivated, with 2 mM glutamine, usually antibiotics (typically pen/strep or gentamicin plus amphotericin B), 10 mM HEPES, 2- mercaptoethanol (0.056 mM)
|Standard conditions (typically 10% DMSO, 90% FBS)