人结肠上皮细胞系HT29-MTX-E12

服务详情

ECACC的 #12040401,HT29-MTX-E12,人结肠上皮细胞系HT29-MTX-E12,1 VIAL,准现货供应原装冻存管。

细胞链接:https://www.culturecollections.org.uk/products/celllines/generalcell/detail.jsp?refId=12040401&collection=ecacc_gc

产品详细信息如下:

ECACC General Cell Collection: HT29-MTX-E12


Supplied by:European Collection of Authenticated Cell Cultures (ECACC)
Culture Type:Cell line
Collection:ECACC General Collection

Catalogue No.:12040401
Cell Line Name:HT29-MTX-E12

Citation Guidance:If use of this culture results in a scientific publication, it should be cited in the publication as: HT29-MTX-E12 (ECACC 12040401)

Keywords:HT29, HT29-MTX, methotrexate, mucous layer, intestinal permeation, oral drug absorption
Cell Line Description:HT29 cells were differentiated into mature goblet cells using methotrexate. Mucous-secreting HT29-MTX subclones were isolated from this cell clone and characterized with regard to tight junction formation, development of confluent monolayers and production of a mucous layer. HT29-MTX-E12 provides a model system to study the influence of the mucous layer on nanoparticle diffusion.
Species:Human
Tissue of Origin:Colon

CellType:Epithelial

Growth Mode:Adherent
DNA Profile:STR-PCR Data:

Amelogenin: X
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6
TPOX: 8,9
vWA: 17


Biosafety Information:Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet

Subculture Routine:Split subconfluent cultures (70-80%) 1:3 to 1:6 using 0.05% trypsin/EDTA; 7.5-10% CO2; 37°C. Suggested seeding density 2-4x10,000 cells/cm².
Culture Medium:DMEM + 2mM Glutamine + 1% Non essential amino acids + 10% Foetal Bovine Serum (FBS).
Depositor:Professor David Brayden, Associate Professor of Drug Delivery and Kevin McMahon, Senior Technical Officer, University College Dublin, UCD School of Veterinary Medicine, Belfield, Dublin 4, IRELAND. Originator: Professor Per Artursson, Uppsala University, Sweden.
Originator:No
Country:Ireland
References:Behrens I, Stenberg P, Artursson P, Kissel T. 2001 Transport of lipophilic drug molecules in a new mucus-secreting cell culture model based on HT29-MTX cells. Pharm Res. 18:1138-45. PMID: 11587485.
Additional Bibliography:Keely S, Rullay A, Wilson C, Carmichael A, Carrington S, Corfield A, Haddleton DM, Brayden DJ. 2005 In vitro and ex vivo intestinal tissue models to measure mucoadhesion of poly (methacrylate) and N-trimethylated chitosan polymers. Pharm Res. 22:38-49. PMID: 15771228.
Patents:None specified by Depositor
Release Conditions:No




ECACC欧洲生物资源库提供经过验证和质量控制的细胞系、核酸和诱导多能干细胞 (iPSC)。并竭诚为研究界提供服务。
ECACC提供超过1100株细胞系,并可为部分细胞系可提供DNARNA等相关产品。






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