合成可逆终止剂 Reversible terminators dNTP-ONH2

合成可逆终止剂Reversible terminators dNTP-ONH2

美国Firebird Biomolecular Sciences 为科研、合成生物学、临床诊断等研究机构单位提供创新试剂。

Firebirddelivers reagent innovations to the research, synthetic biology, and clinicaldiagnostics communities.

Nucleicacid analogs for:

DNAsequencing

Diagnostics

SNPdetection

SyntheticBiology

厂家官网：http://www.firebirdbio.com/

我们提供2种类型的合成可逆终止剂，Reversible terminators dNTP-ONH2：

即用型ONH2，"ready-to-use" (RTU) line ；

肟型（可以用于长期保存的丙酮肟型产品）。

按修饰类型，又可以分为4类：
Ready to use,untagged
Oxime blocked, untagged
Diol linked tags
Disulfide linked tags

Reversible terminators: Oxime blocked, untagged
Reversible terminators with a free 3'-ONH2 are ready to use and do not requiredeprotection or further treatment before use.

Thymine，dTTP-ONH2，5 µmoles $900

Cytosine，dCTP-ONH2，5 µmoles $1050

Adenine，dATP-ONH2，5 µmoles $1050

Guanine，dGTP-ONH2，5 µmoles $1050

Reversible terminators:   Oxime blocked, untagged
Reversible terminators are also available with the 3'-ONH2 protected as theacetoxime, which are more suitable for long term storage than our ready to usereversible terminators. For enzymatic incorporation, the oxime must bedeprotected to the free 3' ONH 2 before use, which can be achieved in situ withbuffered aqueous methoxylamine.

Thymine，货号 TONH2-101，5 µmoles $900
Cytosine，货号 CONH2-102，5 µmoles $1050
Adenine，货号 AONH2-103，5 µmoles $1050
Guanine，货号 GONH2-104，5 µmoles $1050

Reversible terminators: diol linked tags
We offers reversible terminators with a 3'-ONH2 (protected as the acetoxime )and a diol linker carrying a free amino group, to which a tag (fluorescent dyeor other moiety) can be attached. The diol can be rapidly cleaved with diluteaqueous periodate.

Thymine，货号 TONH2-DT，1 µmole $1750
Cytosine，货号 CONH2-DT，1 µmole $1900
Adenine，货号 AONH2-DT，1 µmole $2200
Guanine，货号 GONH2-DT，1 µmole $2300

Reversible terminators: disulfide-linked tags
We offers reversible terminators with a 3'-ONH2 (protected as the acetoxime)and a disulfide linker carrying a free amino group, to which a tag (fluorescentdye or other moiety) can be attached. The disulfide can be rapidly cleaved withphosphine or thiol reagents.Thymine，1 μmole $ 1750
Thymine，货号 TONH2-ST，1 μmole $ 1750
Cytosine，货号 CONH2-ST，1 μmole $ 2000
Adenine，货号 AONH2-ST，1 μmole $ 2400
Guanine，货号 GONH2-ST，1 μmole $ 2500
For enzymatic incorporation, the oxime must be deprotected to the free 3'-ONH2before use, which can be achieved in situ with buffered aqueous methoxylamine.

FirebirdBiomolecular Sciences的其他类型产品介绍：

AEGIS™ Artificially Expanded Genetic InformationSystems

Firebird hascreated different heterocycles in order to implement additional hydrogenbonding patterns for the Z:P and S:B base pairs as shown below. These basepairs are “orthogonal” to C:G and A:T Benner, 2004; Hoshika et al., 2019 ;Sefah et al., 2014; Yang et al., 2006).

AEGIS: dZ and dPPhosphoramidites

dZ Phosphoramidite

Hydrogen bondingpattern: Z

Sugar:2’-Deoxyribose

Heterocycle:Nitropyridine

Linear Formula C50H 57 N 6 O 12 P

Mol Weight 964.99

Cat. No. dZ-PA-101

100 mg $ 720.00

1 gram $5760.00

dP Phosphoramidite

Hydrogen bondingpattern: P

Sugar:2’-Deoxyribose

Heterocycle:Imidazotriazine

Linear Formula C49H 65 N 8 O 7 P

Mol Weight 909.08

Cat. No. dP-PA-102

100 mg $ 850.00

1 gram $6800.00

Oligonucleotidesare available that incorporate dZ and dP.

Ribonucleosidederivatives are also available.

AEGIS: dZ and dPTriphosphates

dZ Triphosphate

Hydrogen bondingpattern: Z

Sugar:2’-deoxyribose

Heterocycle:Nitropyridine

Linear Formula C10 H 16 N 3 O 15 P 3

Mol Weight 511.17

Cat. No. dZTP-101

1 µmole $ 400.00

5 µmoles $ 1600.00

dP Triphosphate

Hydrogen bondingpattern: P

Sugar:2’-deoxyribose

Heterocycle:Imidazotriazine

Linear Formula C10 H 16 N 5 O 13 P 3

Mol Weight 493.15

Cat. No. dPTP-201

1 µmole $ 420.00

5 µmoles $ 1680.00

Ribonucleosidetriphosphates are also available. Please inquire.

AEGIS: dS and dBPhosphoramidites

dS Phosphoramidite

Hydrogen bondingpattern: S

Sugar:2’-Deoxyribose

Heterocycle:Pseudo-C

Linear Formula C47H 54 N 5 O 8 P

Mol Weight 847.93

Cat. No.dS-PA-104S

100 mg $ 720.00

1 gram $5760.00

dB Phosphoramidite

Hydrogen bondingpattern: B

Sugar:2’-Deoxyribose

Heterocycle:Purine

Linear Formula C49H 68 N 7 O 7 P

Mol Weight 898.08

Cat. No.dB-PA-103P

100 mg $ 600.00

1 gram $4800.00

Oligonucleotidesare available that incorporate dS and dB.

Ribonucleosidederivatives are also available.

AEGIS: dS and dBTriphosphates

dS Triphosphate

Hydrogen bondingpattern: S

Sugar:2’-deoxyribose

Heterocycle:Pseudo C

Linear Formula C10 H 18 N 3 O 13 P 3

Mol Weight 481.18

Cat. No. dSTP-401S

1 µmole $ 400.00

5 µmoles $ 1600.00

dB Triphosphate

Hydrogen bondingpattern: B

Sugar:2’-deoxyribose

Heterocycle:Purine

Linear Formula C10 H 16 N 5 O 13 P 3

Mol Weight 507.18

Cat. No. dBTP-301P

1 µmole $ 400.00

5 µmoles $ 1600.00

Ribonucleosidetriphosphates are also available. Please inquire.

SAMRS™ Self Avoiding Molecular Recognition System

Nucleic acidamplification reactions suffer from the formation of off target amplificationproducts. This problem increases with the number of primers in the reaction. Itmainly occurs because of primer primer interactions. Thus, selectively removinghydrogen bonding units from the primer bases results in self avoiding DNAprimers that can be added to enable and improve multiplexed PCR Hoshika et al.,2010)

SAMRS:Phosphoramidites

t* phosphoramidite

1 g $ 1050

10 g $ 8220

a* phosphoramidite

1 g $ 655

10 g $ 5340

c* phosphoramidite

1 g $ 394

10 g $3150

g* phosphoramidite

1 g $ 110

10 g $ 880

Oligonucleotidesare available that incorporate the SAMRS bases.

SAMRS can becombined with AEGIS in oligos to get highly multiplexed and highly clean PCR,as well as isothermal amplification. Please inquire.

BiversalTM nucleotides

The divergencepresent in pathogen genomes makes it difficult or impossible to design a singleprobe or primer that is the exact complement of all possible target sequences.In practice this is handled using degenerate or “universal bases” (e.g.inosine).

Firebirdscientists have created two “ biversals TM . Each exists in two tautomericforms that provide alternate hydrogen bonding patterns. This allows thepyrimidine biversal to pair with either guanine or adenine and the purinebiversal to pair with either thymine or cytosine. These bases provide a newalternative to the

existing methodsof handling divergent sequences for both PCR amplification and SNP detection(Yang et al., 2018).

BiversalPhosphoramidites

Pyrimidine biversal

phosphoramidite

Linear Formula C41H 50 N 5 O 8 P

Mol Weight 771.84

Cat No. Pyr-BiVer

100 mg $ 500

1 gram $ 4000

Purine biversal

phosphoramidite

Linear Formula C50H 62 N 7 O 9 P

Mol Weight 936.04

Cat No. Pur-BiVer

100 mg $ 2850

1 gram Inquire

Firebird sellsoligonucleotides that contain the purine and pyrimidine

biversals. Pleaseinquire.

SNAP2™ Oligonucleotides: Priming with high specificity

Specific DNApriming requires oligonucleotides with complementarity of ≥16 nucleotides but the ability todiscriminate against single mismatches. Dynamic assembly of a primer on a templatecan provide this (Leal et al., 2006).

Controlled poreglass with aldehyde precursor

SNAP2T_HJK001

同时，我们还可以根据客户的需求定制特定修饰连接到伯胺。

孚博生物 优势进口Firebird Biomolecular Sciences的产品，价格优惠，周期短，欢迎咨询！