合成可逆终止剂Reversible terminators dNTP-ONH2
我们提供2种类型的合成可逆终止剂,Reversible terminators dNTP-ONH2:
即用型ONH2,"ready-to-use" (RTU) line ;
肟型(可以用于长期保存的丙酮肟型产品)。
按修饰类型,又可以分为4类:
Ready to use,untagged
Oxime blocked, untagged
Diol linked tags
Disulfide linked tags
Reversible terminators: Oxime blocked, untagged
Reversible terminators with a free 3'-ONH2 are ready to use and do not requiredeprotection or further treatment before use.
Thymine,dTTP-ONH2,5 µmoles $900
Cytosine,dCTP-ONH2,5 µmoles $1050
Adenine,dATP-ONH2,5 µmoles $1050
Guanine,dGTP-ONH2,5 µmoles $1050
Reversible terminators: Oxime blocked, untagged
Reversible terminators are also available with the 3'-ONH2 protected as theacetoxime, which are more suitable for long term storage than our ready to usereversible terminators. For enzymatic incorporation, the oxime must bedeprotected to the free 3' ONH 2 before use, which can be achieved in situ withbuffered aqueous methoxylamine. 
Thymine,5 µmoles $900
Cytosine,5 µmoles $1050
Adenine,5 µmoles $1050
Guanine,5 µmoles $1050
Reversible terminators: diol linked tags
We offers reversible terminators with a 3'-ONH2 (protected as the acetoxime )and a diol linker carrying a free amino group, to which a tag (fluorescent dyeor other moiety) can be attached. The diol can be rapidly cleaved with diluteaqueous periodate.
Thymine,1 µmole $1750
Cytosine,1 µmole $1900
Adenine,1 µmole $2200
Guanine,1 µmole $2300
Reversible terminators: disulfide-linked tags
We offers reversible terminators with a 3'-ONH2 (protected as the acetoxime)and a disulfide linker carrying a free amino group, to which a tag (fluorescentdye or other moiety) can be attached. The disulfide can be rapidly cleaved withphosphine or thiol reagents.
Thymine,1 μmole $ 1750
Cytosine,1 μmole $ 2000
Adenine,1 μmole $ 2400
Guanine,1 μmole $ 2500
For enzymatic incorporation, the oxime must be deprotected to the free 3'-ONH2before use, which can be achieved in situ with buffered aqueous methoxylamine.
同时,我们还可以根据客户的需求定制特定修饰连接到伯胺。详情请咨询我们!