北京孚博生物科技有限公司

原装进口 DSMZ 细胞
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德国DSMZ保藏中心成立于1969年,是德国的国家菌种保藏中心。该中心一直致力于细菌、真菌、质粒、抗菌素、人体和动物细胞、植物病毒等的分类、鉴定和保藏工作。


作为世界上最大的保藏中心之一,DSMZ目前收藏超过70,000种产品,包括约31,000种不同的细菌和6,500种真菌菌株,830种人和动物细胞系,1,500种植物病毒和抗血清,2,000种质粒和噬菌体以及13,000种不同类型的细菌基因组DNA


目前,DSMZ细胞系集合包含从多种组织以及许多杂交瘤中分离出来的840多种灵长类,啮齿动物,两栖动物,鱼类和昆虫来源的永生细胞培养物


The DSMZcell lines collection currently comprises more than 840 different immortalizedcell cultures of primate, rodent, amphibian, fish, and insect origin isolatedfrom numerous tissues as well as a number of hybridomas


厂家官网:https://www.dsmz.de/


孚博生物优势原装进口德国DSMZ 的细胞及配套培养试剂,价格优惠,货期6-8周!
低温产品提供全程干冰运输服务,特殊细胞产品全程液氮运输,质量和售后有保障!



PLB-985细胞产品信息:

ACC 139
Cell line: PLB-985
DSMZ no.: ACC 139
Species: human (Homo sapiens)
Cell type: acute myeloid leukemia (derivative of HL-60)
Origin: allegedly "established from the peripheral blood of a 38-year-oldwoman with acute myeloid leukemia (AML FAB M4) in relapse in 1985"; DNAfingerprinting showed unequivocally that PLB-985 is a subclone of cell lineHL-60
Reference(s): 14491, 15024
Biosafety level: 1
Permissions and restrictions: A

DSMZ Cell Culture Data:
Morphology: single, round cells in suspension; TOIT14_6(`9AEP}XRVV26CN.jpgMedium: 90% RPMI 1640 + 10% h.i. FBS
Subculture: seed out at ca. 1 x 106 cells/ml; maintain at ca.0.5-1 x 106 cells/ml; split 1:2 to 1:5 every 2-3 days; minimumdensity at 0.2 x 106cells/ml
Incubation: at 37 °C with 5% CO2
Doubling time: ca. 30 hours
Harvest: maximal density at about 1.5 x 106 cells/ml
Storage: frozen with 70% medium, 20% FBS, 10% DMSO

DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -; image
Fingerprint: same DNA profile as HL-60 using multiplex PCR of minisatellitemarkers
Species: confirmed originally as human with IEF of AST, MDH and later by STRfingerprinting
Cytogenetics: human near diploid karyotype with 4% polyploidy -46(44-50)<2n>X, -X, +6, dm, dup(1)(q21q41), del(3)(p21), del(6)(q21)x2
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8-, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:

Delivery form


Prices


Frozen culture


400,- €

Growing culture
    (please inquire for exact delivery time)


800,- €

DNA isolated from cell line (25 µg)


500,- €

DNA isolated from cell line (5 µg)


120,- €


产品链接:https://www.dsmz.de/collection/catalogue/details/culture/ACC-139


部分产品列表:


Cell line

DSMZ No

Species

Cell type

LB5871-LYMP

869

human

diffuse large B cell   lymphoma

UPCI-SCC-081

868

human

squamous cell carcinoma

UPCI-SCC-078

867

human

squamous cell carcinoma

UPCI-SCC-077

866

human

squamous cell carcinoma

UPCI-SCC-032

864

human

squamous cell carcinoma

UPCI-SCC-003

863

human

squamous cell carcinoma

INA-6

862

human

multiple myeloma

FAIK3-5

861

murine (mouse)

kidney

JK-6

860

human

multiple myeloma

TT2609-A02

859

human

follicular thyroid carcinoma

CHO-INSR-1284

858

Chinese hamster

ovary cells

NT-27a

857

rat

hepatocellular tumor

N1E-115-1

856

murine (mouse)

neuroblastoma

MUG-MEL2

855

human

melanoma

HEK293-DR-GFP-XRCC3-5/X3+

854

human

embryonal kidney

HEK293-DR-GFP-XRCC2-13/X2+

853

human

embryonal kidney

HEK293-DR-GFP-RAD51D-16/D+

852

human

embryonal kidney

HEK293-DR-GFP-RAD51C-2/C+

851

human

embryonal kidney

HEK293-DR-GFP-RAD51B-9/B+

850

human

embryonal kidney

HEK293-DR-GFP-XRCC3-5

849

human

embryonal kidney

HEK293-DR-GFP-XRCC2-13

848

human

embryonal kidney

HEK293-DR-GFP-RAD51D-16

847

human

embryonal kidney

HEK293-DR-GFP-RAD51C-2

846

human

embryonal kidney

HEK293-DR-GFP-RAD51B-9

845

human

embryonal kidney

HEK293-DR-GFP

844

human

embryonal kidney

CL-52b

843

rat

hepatocellular carcinoma

U2OS#18-XRCC3-6A/X3+

842

human

osteosarcoma

U2OS#18-XRCC2-5E/X2+

841

human

osteosarcoma

U2OS#18-RAD51D-4/D+

840

human

osteosarcoma

U2OS#18-RAD51C-15/C+

839

human

osteosarcoma

U2OS#18-RAD51B-8/B+

838

human

osteosarcoma

U2OS#18-XRCC3-6A

837

human

osteosarcoma

U2OS#18-XRCC2-5E

836

human

osteosarcoma

U2OS#18-RAD51D-4

835

human

osteosarcoma

U2OS#18-RAD51C-15

834

human

osteosarcoma

U2OS#18-RAD51B-8

833

human

osteosarcoma

U2OS#18

832

human

osteosarcoma

EL-4

831

murine (mouse)

T cell lymphoma

CHP-100

830

human

neuroepithelioma

JVE-367

829

human

colon carcinoma

MCA-B1

828

canine (dog)

acanthomatous epulis

IPH-926

827

human

breast carcinoma

JVE-528

826

human

colon carcinoma

JVE-371

825

human

colon carcinoma


KMST-6-TNF

824

human

fibroblasts

JVE-253

823

human

colon carcinoma

JVE-241

822

human

colon carcinoma

JVE-222

821

human

colon carcinoma

JVE-207

820

human

colon carcinoma

JVE-192

819

human

colon carcinoma

JVE-187

818

human

colon carcinoma

CX-03

816

human

uterine carcinosarcoma

MCC-60

815

human

colorectal cancer

MAPAC-HS-77

814

human

pancreatic cancer

JVE-127

813

human

colon carcinoma

JVE-114

812

human

colon carcinoma

GH

811

human

testicular germ cell tumor

CCC-5

810

human

cholangiocellular carcinoma

JVE-109

809

human

colon carcinoma

KP-363T

808

human

colon carcinoma

JVE-103

807

human

colon carcinoma

JVE-059

806

human

colon carcinoma

538-MG-BA

805

human

glioblastoma

170-MG-BA

804

human

glioblastoma

JVE-017

803

human

colon carcinoma

GL-261

802

murine (mouse)

glioblastoma

MBU-TS4

801

human

keratinocytes

MBU-TS1

800

human

keratinocytes

MBU-T

799

human

keratinocytes

ETCC-016

798

human

lung adenocarcinoma

ETCC-011

797

human

breast carcinoma

MBU-IM

796

human

keratinocytes

U-2946

795

human

B cell lymphoma

PCL-12

794

human

chronic B cell leukemia

ETCC-010

793

human

breast ductal carcinoma

ETCC-008

792

human

breast ductal carcinoma

ETCC-007

791

human

breast ductal carcinoma

ETCC-006

790

human

breast ductal carcinoma

BLN-7

789

human

glioblastoma

JIH-5

788

human

acute biphenotypic leukemia

BLN-3

787

human

glioblastoma

JVE-015

786

human

colon carcinoma

U-2-OS

785

human

osteosarcoma

FADU

784

human

pharynx carcinoma

PANC-1

783

human

pancreas carcinoma

NALM-33

782

human

B cell precursor leukemia

HS-578T

781

human

breast carcinoma

HC-11

780

murine (mouse)

normal mammary epithelial

WSU-DLCL

779

human

B cell lymphoma

LOPRA-1

778

human

multiple myeloma

MOLM-14

777

human

acute myeloid leukemia

PG-EBV

776

human

B lymphoblastoid cells


UOC-M1

775

human

acute myeloid leukemia

232A4

774

human

B lymphoblastoid cells

CII

773

human

chronic B cell leukemia

L4.5

772

murine (mouse)

derivative of L-929

CI

770

human

chronic B cell leukemia

WA-C3CD5+

769

human

chronic B cell leukemia

U-2904

768

human

B cell lymphoma

WA-OSEL

767

human

chronic B cell leukemia

PGA-1

766

human

chronic B cell leukemia

HG-3

765

human

chronic B cell leukemia

I83-LCL

764

human

B lymphoblastoid cells (from

patient   with chronic B cell

I83-E95

763

human

chronic B cell leukemia

HAIR-M

762

human

hairy cell leukemia

OCI-LY3

761

human

B cell lymphoma

BXPC-3

760

human

pancreas carcinoma

LS-174T

759

human

colon adenocarcinoma

SK-MM-1

758

human

plasma cell leukemia

BJAB

757

human

Burkitt lymphoma

R-06E

756

Egyptian   fruit bat

embryonal

MUG-CHOR-1

755

human

chordoma

R-05T

754

Egyptian   fruit bat

fetal   brain

PACADD-188

753

human

pancreas carcinoma

1618-K

752

human

testicular germ cell tumor

PACADD-183

751

human

pancreas carcinoma

PACADD-165

750

human

pancreas carcinoma

U-CH2

749

human

chordoma

U-CH1

748

human

chordoma

SUSA

747

human

testicular germ cell tumor

PACADD-161

746

human

pancreas carcinoma

PACADD-159

745

human

pancreas carcinoma

JOPACA-1

744

human

pancreas carcinoma

GCT-27

743

human

testicular germ cell tumor

BALL-1

742

human

B cell leukemia

17.94

741

human

Wilms´ tumor

HD-MB03

740

human

medulloblastoma

T-47D

739

human

breast carcinoma

MDA-MB-468

738

human

breast carcinoma

NCI-H460

737

human

lung carcinoma

SK-BR-3

736

human

breast carcinoma

HT-93

735

human

acute myeloid leukemia

CALU-6

734

human

anaplastic carcinoma

MIA-PACA-2

733

human

pancreas carcinoma

MDA-MB-231

732

human

breast carcinoma

CAKI-1

731

human

kidney carcinoma

OCI-AML6

730

human

acute myeloid leukemia






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