德国DSMZ保藏中心成立于1969年，是德国的国家菌种保藏中心。该中心一直致力于细菌、真菌、质粒、抗菌素、人体和动物细胞、植物病毒等的分类、鉴定和保藏工作。
作为世界上最大的保藏中心之一，DSMZ目前收藏超过70,000种产品，包括约31,000种不同的细菌和6,500种真菌菌株，830种人和动物细胞系，1,500种植物病毒和抗血清，2,000种质粒和噬菌体以及13,000种不同类型的细菌基因组DNA。
目前，DSMZ细胞系集合包含从多种组织以及许多杂交瘤中分离出来的840多种灵长类，啮齿动物，两栖动物，鱼类和昆虫来源的永生细胞培养物
The DSMZcell lines collection currently comprises more than 840 different immortalizedcell cultures of primate, rodent, amphibian, fish, and insect origin isolatedfrom numerous tissues as well as a number of hybridomas
厂家官网：https://www.dsmz.de/
孚博生物优势原装进口德国DSMZ 的细胞及配套培养试剂，价格优惠，货期6-8周！
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PLB-985细胞产品信息：
ACC 139
Cell line: PLB-985
DSMZ no.: ACC 139
Species: human (Homo sapiens)
Cell type: acute myeloid leukemia (derivative of HL-60)
Origin: allegedly "established from the peripheral blood of a 38-year-oldwoman with acute myeloid leukemia (AML FAB M4) in relapse in 1985"; DNAfingerprinting showed unequivocally that PLB-985 is a subclone of cell lineHL-60
Reference(s): 14491, 15024
Biosafety level: 1
Permissions and restrictions: A
DSMZ Cell Culture Data:
Morphology: single, round cells in suspension; 
Medium: 90% RPMI 1640 + 10% h.i. FBS
Subculture: seed out at ca. 1 x 106 cells/ml; maintain at ca.0.5-1 x 106 cells/ml; split 1:2 to 1:5 every 2-3 days; minimumdensity at 0.2 x 106cells/ml
Incubation: at 37 °C with 5% CO2
Doubling time: ca. 30 hours
Harvest: maximal density at about 1.5 x 106 cells/ml
Storage: frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
Mycoplasma:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology:
CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -; image
Fingerprint: same DNA profile as HL-60 using multiplex PCR of minisatellitemarkers
Species: confirmed originally as human with IEF of AST, MDH and later by STRfingerprinting
Cytogenetics: human near diploid karyotype with 4% polyploidy -46(44-50)<2n>X, -X, +6, dm, dup(1)(q21q41), del(3)(p21), del(6)(q21)x2
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8-, HIV-1 -, HIV-2 -, HTLV-I/II -, MLV -, SMRV -
Supplied as:
Delivery form |
| Prices |
|
Frozen culture |
| 400,- € |
Growing culture
(please inquire for exact delivery time) |
| 800,- € |
DNA isolated from cell line (25 µg) |
| 500,- € |
DNA isolated from cell line (5 µg) |
| 120,- € |
产品链接：https://www.dsmz.de/collection/catalogue/details/culture/ACC-139
部分产品列表：
Cell line | DSMZ No | Species | Cell type |
LB5871-LYMP | 869 | human | diffuse large B cell lymphoma |
UPCI-SCC-081 | 868 | human | squamous cell carcinoma |
UPCI-SCC-078 | 867 | human | squamous cell carcinoma |
UPCI-SCC-077 | 866 | human | squamous cell carcinoma |
UPCI-SCC-032 | 864 | human | squamous cell carcinoma |
UPCI-SCC-003 | 863 | human | squamous cell carcinoma |
INA-6 | 862 | human | multiple myeloma |
FAIK3-5 | 861 | murine (mouse) | kidney |
JK-6 | 860 | human | multiple myeloma |
TT2609-A02 | 859 | human | follicular thyroid carcinoma |
CHO-INSR-1284 | 858 | Chinese hamster | ovary cells |
NT-27a | 857 | rat | hepatocellular tumor |
N1E-115-1 | 856 | murine (mouse) | neuroblastoma |
MUG-MEL2 | 855 | human | melanoma |
HEK293-DR-GFP-XRCC3-5/X3+ | 854 | human | embryonal kidney |
HEK293-DR-GFP-XRCC2-13/X2+ | 853 | human | embryonal kidney |
HEK293-DR-GFP-RAD51D-16/D+ | 852 | human | embryonal kidney |
HEK293-DR-GFP-RAD51C-2/C+ | 851 | human | embryonal kidney |
HEK293-DR-GFP-RAD51B-9/B+ | 850 | human | embryonal kidney |
HEK293-DR-GFP-XRCC3-5 | 849 | human | embryonal kidney |
HEK293-DR-GFP-XRCC2-13 | 848 | human | embryonal kidney |
HEK293-DR-GFP-RAD51D-16 | 847 | human | embryonal kidney |
HEK293-DR-GFP-RAD51C-2 | 846 | human | embryonal kidney |
HEK293-DR-GFP-RAD51B-9 | 845 | human | embryonal kidney |
HEK293-DR-GFP | 844 | human | embryonal kidney |
CL-52b | 843 | rat | hepatocellular carcinoma |
U2OS#18-XRCC3-6A/X3+ | 842 | human | osteosarcoma |
U2OS#18-XRCC2-5E/X2+ | 841 | human | osteosarcoma |
U2OS#18-RAD51D-4/D+ | 840 | human | osteosarcoma |
U2OS#18-RAD51C-15/C+ | 839 | human | osteosarcoma |
U2OS#18-RAD51B-8/B+ | 838 | human | osteosarcoma |
U2OS#18-XRCC3-6A | 837 | human | osteosarcoma |
U2OS#18-XRCC2-5E | 836 | human | osteosarcoma |
U2OS#18-RAD51D-4 | 835 | human | osteosarcoma |
U2OS#18-RAD51C-15 | 834 | human | osteosarcoma |
U2OS#18-RAD51B-8 | 833 | human | osteosarcoma |
U2OS#18 | 832 | human | osteosarcoma |
EL-4 | 831 | murine (mouse) | T cell lymphoma |
CHP-100 | 830 | human | neuroepithelioma |
JVE-367 | 829 | human | colon carcinoma |
MCA-B1 | 828 | canine (dog) | acanthomatous epulis |
IPH-926 | 827 | human | breast carcinoma |
JVE-528 | 826 | human | colon carcinoma |
JVE-371 | 825 | human | colon carcinoma |
KMST-6-TNF | 824 | human | fibroblasts |
JVE-253 | 823 | human | colon carcinoma |
JVE-241 | 822 | human | colon carcinoma |
JVE-222 | 821 | human | colon carcinoma |
JVE-207 | 820 | human | colon carcinoma |
JVE-192 | 819 | human | colon carcinoma |
JVE-187 | 818 | human | colon carcinoma |
CX-03 | 816 | human | uterine carcinosarcoma |
MCC-60 | 815 | human | colorectal cancer |
MAPAC-HS-77 | 814 | human | pancreatic cancer |
JVE-127 | 813 | human | colon carcinoma |
JVE-114 | 812 | human | colon carcinoma |
GH | 811 | human | testicular germ cell tumor |
CCC-5 | 810 | human | cholangiocellular carcinoma |
JVE-109 | 809 | human | colon carcinoma |
KP-363T | 808 | human | colon carcinoma |
JVE-103 | 807 | human | colon carcinoma |
JVE-059 | 806 | human | colon carcinoma |
538-MG-BA | 805 | human | glioblastoma |
170-MG-BA | 804 | human | glioblastoma |
JVE-017 | 803 | human | colon carcinoma |
GL-261 | 802 | murine (mouse) | glioblastoma |
MBU-TS4 | 801 | human | keratinocytes |
MBU-TS1 | 800 | human | keratinocytes |
MBU-T | 799 | human | keratinocytes |
ETCC-016 | 798 | human | lung adenocarcinoma |
ETCC-011 | 797 | human | breast carcinoma |
MBU-IM | 796 | human | keratinocytes |
U-2946 | 795 | human | B cell lymphoma |
PCL-12 | 794 | human | chronic B cell leukemia |
ETCC-010 | 793 | human | breast ductal carcinoma |
ETCC-008 | 792 | human | breast ductal carcinoma |
ETCC-007 | 791 | human | breast ductal carcinoma |
ETCC-006 | 790 | human | breast ductal carcinoma |
BLN-7 | 789 | human | glioblastoma |
JIH-5 | 788 | human | acute biphenotypic leukemia |
BLN-3 | 787 | human | glioblastoma |
JVE-015 | 786 | human | colon carcinoma |
U-2-OS | 785 | human | osteosarcoma |
FADU | 784 | human | pharynx carcinoma |
PANC-1 | 783 | human | pancreas carcinoma |
NALM-33 | 782 | human | B cell precursor leukemia |
HS-578T | 781 | human | breast carcinoma |
HC-11 | 780 | murine (mouse) | normal mammary epithelial |
WSU-DLCL | 779 | human | B cell lymphoma |
LOPRA-1 | 778 | human | multiple myeloma |
MOLM-14 | 777 | human | acute myeloid leukemia |
PG-EBV | 776 | human | B lymphoblastoid cells |
UOC-M1 | 775 | human | acute myeloid leukemia |
232A4 | 774 | human | B lymphoblastoid cells |
CII | 773 | human | chronic B cell leukemia |
L4.5 | 772 | murine (mouse) | derivative of L-929 |
CI | 770 | human | chronic B cell leukemia |
WA-C3CD5+ | 769 | human | chronic B cell leukemia |
U-2904 | 768 | human | B cell lymphoma |
WA-OSEL | 767 | human | chronic B cell leukemia |
PGA-1 | 766 | human | chronic B cell leukemia |
HG-3 | 765 | human | chronic B cell leukemia |
I83-LCL | 764 | human | B lymphoblastoid cells (from patient with chronic B cell |
I83-E95 | 763 | human | chronic B cell leukemia |
HAIR-M | 762 | human | hairy cell leukemia |
OCI-LY3 | 761 | human | B cell lymphoma |
BXPC-3 | 760 | human | pancreas carcinoma |
LS-174T | 759 | human | colon adenocarcinoma |
SK-MM-1 | 758 | human | plasma cell leukemia |
BJAB | 757 | human | Burkitt lymphoma |
R-06E | 756 | Egyptian fruit bat | embryonal |
MUG-CHOR-1 | 755 | human | chordoma |
R-05T | 754 | Egyptian fruit bat | fetal brain |
PACADD-188 | 753 | human | pancreas carcinoma |
1618-K | 752 | human | testicular germ cell tumor |
PACADD-183 | 751 | human | pancreas carcinoma |
PACADD-165 | 750 | human | pancreas carcinoma |
U-CH2 | 749 | human | chordoma |
U-CH1 | 748 | human | chordoma |
SUSA | 747 | human | testicular germ cell tumor |
PACADD-161 | 746 | human | pancreas carcinoma |
PACADD-159 | 745 | human | pancreas carcinoma |
JOPACA-1 | 744 | human | pancreas carcinoma |
GCT-27 | 743 | human | testicular germ cell tumor |
BALL-1 | 742 | human | B cell leukemia |
17.94 | 741 | human | Wilms´ tumor |
HD-MB03 | 740 | human | medulloblastoma |
T-47D | 739 | human | breast carcinoma |
MDA-MB-468 | 738 | human | breast carcinoma |
NCI-H460 | 737 | human | lung carcinoma |
SK-BR-3 | 736 | human | breast carcinoma |
HT-93 | 735 | human | acute myeloid leukemia |
CALU-6 | 734 | human | anaplastic carcinoma |
MIA-PACA-2 | 733 | human | pancreas carcinoma |
MDA-MB-231 | 732 | human | breast carcinoma |
CAKI-1 | 731 | human | kidney carcinoma |
OCI-AML6 | 730 | human | acute myeloid leukemia |
Medium: 90% RPMI 1640 + 10% h.i. FBS